Controlling system of teleostean spermatogenesis

    We established the culture system of the medaka testis in which every step of spermatogenesis may progress under the control through the germ-somatic cell interaction (Iwasaki et al., 2009). In this system, primary spermatocytes differentiate into swimming sperm. We also established germ cell culture in which primary spermatocytes autonomously differentiated into fertilizable sperm (Saiki et al., 1997; Sasaki et al., 2005). We are investigating the mechanisms for controlling spermatogenesis using those culture systems at cellular and molecular levels and straggling to establish a system for estimating the influences of environment, such as endocrine disruptors in freshwater or microgravity in space, in spermatogenesis of the medaka fish and its control.



Iwasaki Y, Ohkawa K, Sadakata H, Kashiwadate A, Takayama-Watanabe E, Onitake K, Watanabe A. 2009. Develop. Growth Differ. 51: 521-532.
Sasaki T, Watanabe A, Takayama-Watanabe E,  Suzuki M,  Abe H,  Onitake K. 2005. Develop. Growth & Differ. 47: 87-97.


 Publications       Back